It is difficult to say what the problem is but you can try to sort the problems one by one.
1. You mentioned that the lens is sharp in most other cases and that you never had a problem in any other shots. Does this count for all focal lenghts?
2. You used a tripod and the IS was left on. In Bryan´s review of this lens he says:
This IS version is tripod sensing to prevent feedback loops between the IS sensor and stabilizer motor vibrations. The manual recommends turning IS off when tripod-mounting the lens (to save battery life) or when panning (panning mode IS is not available).
This could be the problem. I do not think movements from the tripod itself is the problem.
3. You said "when i shoot portraits at the wide end of the 17-55 2.8, I'm getting horribly unsharp pictures". Is this a problem only when shooting portraits or in general?
4. Is this a crop from the center of the image or from the side? If it is from the left or right side is the center sharp/in focus?
5. You were using a flash. Have you tried shooting the same portraits without the flash?
These are only a few thoughts. It is difficult to guess. I would have performed a few test with the camera on the tripod, no flash, IS off, plenty of light, using center focus point only and using different focal lengths. That will give you a clue if it is the lens or not.
- Johnny




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