I started with a LowePro Nature Trekker and upgraded a couple years later to a Photo Trekker (the other bag became a hand-me-down to my wife). Both are still going strong, though due to massive differences in torso I just can't wear her bag for long if at all. Backpacks have become a means of transportation for us; we each use a Shootsac for a lot of on-the-go lens work, or I use a LowePro vest and belt with any assortment of LowePro LensExchange cases and Utility Pouch for field work.
I recently picked up a LowePro TopLoader 75 for the times when I want to take just one camera and one lens in the car, and don't want it banging around. I've also added a Pelican 1610 to the arsenal for more varied hauling needs.