Originally Posted by mikehillman89
The difference is likely due to a different aperture between shots.
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Originally Posted by csphua
Look through the lens (not mounted on the camera), held up to a light - do you see anything in there? If there's internal fungus, you'll see it.
Do you have more than one lens? If so, take a picture of something featureless (a clear blue sky, a flat white wall, etc.) with each lens, in both cases at the narrowest aperture (largest f-number, e.g. f/22). If the spots are in the same place, it's a dirty sensor. To me, what you're seeing looks like a dirty sensor. Just blowing off the sensor is not enough, sometimes. Wet cleaning might be required, and you may want to have that professionally done (to see what's involved, you can read Bryan's tip on Sensor Cleaning).
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