Originally Posted by
Michael G. Clark
When using Canon DSLRS. it is best to avoid the '+1/3 stop' ISO settings (125, 250, 500, 1000, 2000, etc.) Due to the way the sensor is set at whole stop settings and the exposure/development is 'pulled/pushed' you wind up losing 1/3 stop in the highlights and increasing the noise in the shadows. It's basically the same as exposing to the left and then pushing to the right in development.
If not burning the highlights and getting the widest dynamic range possible is your primary concern, use whole stop ISO settings (100, 200, 400, 800, etc.).
If shadow detail is the primary concern, use '-1/3 stop' settings (160, 320, 640, 1250, etc.). You give up 1/3 stop of DR, but gain detail in the shadows the same as if you "ETTR" by 1/3 stop.
In several tests done by various folks, under certain conditions ISO 125 can be noisier than ISO 1250!