Rigidly mounting an odd combination: T2i + 85L f/1.2

[ChadS]

@HD You're exactly correct. DoF goes as the 1 / magnification squared (basically). so at macro distances the DoF would be insanely thin - and we will repeat this entire project with just such a lens once we've got everything else working. However, for now the customer wants a substantially larger interrogation region than could be imaged at 1:1. Instead, the 0.1x mag of the 85mm lens works out almost perfectly. Were I to use the 180 at the same framing the DoF would be substantially thicker.

DoF is not my only issue. If I just needed thin DoF I'd use a 1000x microscope objective. And while cool, those beasties don't allow for much field of view. Thanks for the help though, fresh eyes are always appreciated.

[neuroanatomist]

If I just needed thin DoF I'd use a 1000x microscope objective.

Wow - where'd you get one of those? My highest power objectives for routine use are 100x NA=1.4 and require oil immersion, although I do have a 150x NA=1.35 glycerol immersion lens, and a 150x NA=0.95 dry lens for materials work.

[HDNitehawk]

Wow - where'd you get one of those? My highest power objectives for routine use are 100x NA=1.4 and require oil immersion, although I do have a 150x NA=1.35 glycerol immersion lens, and a 150x NA=0.95 dry lens for materials work.

Where can I get these and...do they have an adapter so I can hook it up to my 5D II

[ChadS]

Where can I get these and...do they have an adapter so I can hook it up to my 5D II

http://www.edmundoptics.com/products...categoryid=172
Edmund is sort of like the Walmart or Target of optical supplies. They have everything but not necessarily the high-end stuff. There are 100x objectives for Mitutoyo infinity-corrected objectives and several in the finites.

As to mounting your 5dII? Most microscopes these days have some sort of camera port that you can use instead of an eyepiece. Think your current lenses are expensive? Check out the prices of the good objectives - $21k for an 80X UV objective and that's for something that weighs about a pound.